The Host-Parasite Interactions of Varroa Mites and Honey Bees: Varroa Mite Feeding

Thursday, 25 September 2025

As beekeepers we often see the damage inflected by Varroa mites (Varroa destructor) at the colony level. When colonies are not managed vigilantly for Varroa mites, and populations of mites reach a critical level, they colony is significantly weakened and is at high risk of dying before or during the winter months. To better understand why Varroa mites cause colony loss it is important to explore the biology of Varroa mites including their host-parasite interaction. This week’s blog will specifically focus of Varroa mite feeding and the direct impacts feeding has on their honey bee host.

The Host-Parasite Interactions of Varroa Mites and Honey Bees: Varroa Mite Feeding

Varroa mites remain the most widespread and economically damaging pest of honey bees1. It is important to continue researching how Varroa mites target and parasitize honey bees to develop new chemical treatments and improve management practices. An aspect of Varroa mite biology that is worthwhile to discuss is how their feeding practices directly impact honey bees.

Varroa parasitizes both the adult bee and developing brood by feeding on both hemolymph and fat bodies. However, more recent studies have identified that the fat body is the primary feeding source for Varroa mites as this tissue plays a critical role for honey bee immune function, pesticide detoxification, energy metabolism, and overall health of the honey bee2. This means that damage inflicted by Varroa mites to the fat body impacts the bee’s ability to protect itself from pathogens such as viruses and bacteria, and to detoxify pesticides, which overall weakens the bee and shortens their lifespan2.

Figure 1. Research by Ramsey et al. (2019) demonstrates the preferred feeding location of Varroa destructor on honey bee hosts, with the majority of mites (95.2%) feeding underneath the metasoma (an area predominated by fat body tissue) (n = 104) (Diagram created by Ramsey et al. 2019).

Beekeepers should consider the fact that the preferred feeding location of Varroa mites (underneath the metasoma) is in an area not visible to them when inspecting colonies. Therefore, when a beekeeper does identify a mite dorsally (on top) of a bee, this often indicates the ventral (underneath) feeding locations are already occupied by additional mites, and mite levels may be critically high within the colony.

The feeding process involves puncturing the bee’s cuticle using toothed chelicerae, and then using their pharyngeal pump to extract nutrients1. Continuous feeding weakens the host, reduces longevity, and facilitates viral infections1.

Exploring other impacts of Varroa mite feeding, brood infested with mites leads to a reduction in the body weight of newly emerged bees and malformed adult bees, which indicates developmental stress and compromised health3. Reduced body weight and malformation is caused by a combination of loss of nutrients, viral transmission and suppressed immune function during development3. Bees with compromised development have a reduced lifespan, which contributes to weakened colonies and overall colony decline.

One of the primary ways Varroa mite feeding directly impacts honey bees is their saliva composition. The saliva of Varroa mites contains a complex mixture of bioactive proteins and enzymes that disrupt host immunity and wound healing4. Typically, when an insect’s cuticle is punctured the wound will naturally heal to prevent hemolymph loss. However, when Varroa punctures the cuticle of a honey bee the wound inflicted does not close properly suggesting that the saliva interferes with normal healing4. Approximately 356 proteins have been identified in the saliva of Varroa mites that can either interfere with the immune response to heal wounds, or function as virulence factors, enhancing the mite’s ability to infect the honey bee host5. Furthermore, the saliva of mites contains various enzymes which protect the mite against harmful compounds produced by the honey bee during feeding2,6. These enzymes break down toxic compounds that are produced during feeding, and other harmful compounds existing within the honey bee host, which provides the Varroa mites an adaptive advantage to parasitism2,6.

The direct damage inflicted by the Varroa mite through feeding is severe, and contributes to weakened colonies and colony loss. However, there are other aspects of the host-parasite interaction between Varroa mites and honey bees that contribute to the overall colony decline associated with high Varroa mite levels. Next week’s blog will further explore Varroa mite biology and how this parasite transmits viruses to their host.

References
  1. Jeyapriya, G., Sumathi, E., Saminathan, V.R., Renukadevi, P., Sasikala, R., Priya, S.S., Kowsika, S. and Pradeep, S., 2025. Parasitic Mites of Honey Bees (Apis Spp.): A Detailed Review of Varroa destructor in Parasitism, Pathogen Transmission and its Management. Acta Parasitologica70(5), pp.1-25.
  2. Ramsey, S.D., Ochoa, R., Bauchan, G., Gulbronson, C., Mowery, J.D., Cohen, A., Lim, D., Joklik, J., Cicero, J.M., Ellis, J.D. and Hawthorne, D., 2019. Varroa destructor feeds primarily on honey bee fat body tissue and not hemolymph. Proceedings of the National Academy of Sciences116(5), pp.1792-1801.
  3. Martin, S.J., 2001. The role of Varroa and viral pathogens in the collapse of honeybee colonies: a modelling approach. Journal of Applied Ecology38(5), pp.1082-1093.
  4. Kanbar, G. and Engels, W.J.P.R., 2003. Ultrastructure and bacterial infection of wounds in honey bee (Apis mellifera) pupae punctured by Varroa mites. Parasitology research90(5), pp.349-354.
  5. Zhang, Y. and Han, R., 2019. Insight into the salivary secretome of Varroa destructor and salivary toxicity to Apis cerana. Journal of Economic Entomology112(2), pp.505-514.
  6. Morfin, N., Goodwin, P.H. and Guzman-Novoa, E., 2023. Varroa destructor and its impacts on honey bee biology. Frontiers in Bee Science1, p.1272937.

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